2024-03-29T10:18:32Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00001606
2022-12-15T03:34:16Z
432:477:478
453:454
Capacity of dental pulp differentiation after tooth transplantation
Ogawa, Ryoichiro
Saito, Chikara
Jung, Han-Sung
Ohshima, Hayato
Springer-Verlag 2006
auto-graft
bone development
dental pulp
mouse
tooth transplantation
Dental pulp elaborates both bone and dentin matrix under pathological conditions, where the contribution of periodontal tissue to this event cannot be excluded. This study aimed to clarify the capability of dental pulp to deposit bone matrix through an auto-graft experiment using immunohistochemistry for 5-bromo-2’-deoxyuridine (BrdU) and nestin, and histochemistry for tartrate-resistant acid phosphatase (TRAP). Following the extraction of the molars of 3-week-old mice, the roots and pulp floor were resected and immediately transplanted into the sublingual region. On Days 5-7, tubular dentin formation commenced next to the preexisting dentin at the pulp horn where nestin-positive odontoblast-like cells were arranged. Until Day 14, bone-like tissue formation occurred in the pulp chamber, where intense TRAP-positive cells appeared. These results suggest that the odontoblast- and osteoblast-lineage cells reside in the dental pulp. Overall, specific dental pulp regeneration will provide fundamental knowledge for the realization of human tooth regeneration in the near future.
Springer Berlin/Heidelberg
2006-12
eng
journal article
http://hdl.handle.net/10191/1805
https://niigata-u.repo.nii.ac.jp/records/1606
info:doi/10.1007/s00441-006-0242-0
AA00600105
0302766X
Cell and tissue research
Cell and tissue research
326
3
715
724
https://niigata-u.repo.nii.ac.jp/record/1606/files/9_0006.pdf
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2019-07-29