2024-03-28T12:38:28Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00001564
2022-12-15T04:23:27Z
453:454
485:486:487
Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins
Mikami, Satoshi
Kishimoto, Tadashi
Hori, Hidetaka
Mitsui, Toshiaki
Oryza sativa L.
membrane protein
2D-PAGE
NEPHGE
Cytosolic and membrane-associated proteins prepared from rice cells were separated and compared by two different 2D-PAGE methods, isoelectric focusing (IEF)/SDS-PAGE and nonequilibrium pH gradient electrophoresis (NEPHGE)/SDS-PAGE. Although IEF/SDS-PAGE of the cytosolic proteins showed sufficient resolution, some mitochondrial and basic microsomal membrane-associated proteins were weakly or hardly detectable on the 2D gel. High-quality and -quantity separation of the organelle membrane-associated proteins was accomplished by NEPHGE/SDS-PAGE, the advantage of this method being more critical in tightly membrane-bound proteins that were unwashable with NaCl. These results indicate that NEPHGE/SDS-PAGE is a useful tool for the proteomic analysis of rice membrane-associated proteins.
Japan Society for Bioscience, Biotechnology, and Agrochemistry
2002
eng
journal article
VoR
http://hdl.handle.net/10191/6327
https://niigata-u.repo.nii.ac.jp/records/1564
http://doi.org/10.1271/bbb.66.1170
AA10824164
0916-8451
Bioscience, Biotechnology, and Biochemistry
66
5
1170
1173
https://niigata-u.repo.nii.ac.jp/record/1564/files/07_0003.pdf
application/pdf
1.0 MB
2019-07-29