2024-03-29T05:47:48Z
https://niigata-u.repo.nii.ac.jp/oai
oai:niigata-u.repo.nii.ac.jp:00001497
2022-12-15T04:15:19Z
432:477:478
453:454
An Ultrastructural Study of Cell-Cell Contact between Mouse Spleen Cells and Calvaria-Derived Osteoblastic Cells in a Co-Culture System for Osteoclast Formation
Amizuka, Norio
Takahashi, Naoyuki
Udagawa, Nobuyuki
Suda, Tatsuo
Ozawa, Hidehiro
Co-culture
Osteoclast formation
Osteoblastic cells
Cell-cell contact
Microenvironment
We have previously established a mouse co-culture system of osteoblastic cells and spleen cells for examining osteoclast differentiation. In the present study, we examined the morphological features of the cell-cell contact between mouse spleen cells and osteoblastic cells in the co-cultures. Light microscopic investigations revealed that tartrate-resistant acid phosphatase (TRAP)positive mononuclear and multinucleated spleen cells appeared in the vicinity of alkaline phosphatase (ALP)-positive osteoblastic cells. Ultrastructurally, spleen cells extended long cytoplasmic filopodia in all directions, by which spleen cells touched adjacent osteoblastic cells. The adjacent osteoblastic cells and spleen cells adhered to each other by forming electron-dense cytoplasmic materials on their inner leaflets of plasma membranes at cell-cell contact sites. Some adjacent osteoblastic and spleen cells made contact on the plasma membranes, forming an extracellular microenvironment. Coated pits were also formed on the plasma membranes facing this microenvironment. These morphological features of the cell-cell contact between osteoblastic cells and spleen cells indicate that there is internalization of organic components, i.e., receptor-mediated endocytosis in the contact sites between the two types of cells.
日本組織細胞化学会
1997
eng
journal article
VoR
http://hdl.handle.net/10191/5698
https://niigata-u.repo.nii.ac.jp/records/1497
http://doi.org/10.1267/ahc.30.351
AA00508022
0044-5991
Acta histochemica et cytochemica
30
4
351
362
https://niigata-u.repo.nii.ac.jp/record/1497/files/7_0003.pdf
application/pdf
3.3 MB
2019-07-29