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Distributional Changes of Lysosomes in In Vitro Matured and Fertilized Porcine Oocytes Visualized by Acridine Orange Staining
http://hdl.handle.net/10191/25133
http://hdl.handle.net/10191/2513364632e61-cb5a-468a-ad39-b7d5acbc2a9d
名前 / ファイル | ライセンス | アクション |
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26_1_48-53.pdf (253.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-02-12 | |||||
タイトル | ||||||
タイトル | Distributional Changes of Lysosomes in In Vitro Matured and Fertilized Porcine Oocytes Visualized by Acridine Orange Staining | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Distributional Changes of Lysosomes in In Vitro Matured and Fertilized Porcine Oocytes Visualized by Acridine Orange Staining | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Porcine oocyte | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Maturation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fertilization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lysosome | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Acridine orange | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Yamazaki, Shoko
× Yamazaki, Shoko× Niimura, Sueo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The distribution of lysosomes during porcine oocyte maturation and fertilization in vitro was revealed by using acridine orange staining. The lysosomal distribution was classified into three types: generally even distribution throughout the ooplasm (Type I), less distribution in the peripheral ooplasm (Type II), and less distribution in the peripheral and inner ooplasm (Type III). All oocytes examined at 0 and 8 h after maturation culture showed the Type I lysosome distribution and 97% were at the germinal vesicle (GV) stage. When cultured for 22 h, the relative abundance of Type I oocytes decreased to 58%, while Type II and Type III oocytes appeared at relative abundances of 35 and 8%, respectively. After 32 and 44 h of culture, 35 and 80% of the oocytes, respectively, were Type III. When cultured with olomoucine, IBMX or dbcAMP for 22 h, 100, 79 and 94% of the oocytes, respectively, showed the Type I distribution of lysosomes, and their nuclei were almost all at the GV stage (100, 93 and 100%). The results of the present study suggest that there may be a close relationship between nuclear maturation and the distribution of lysosomes in the cytoplasm, and that the distribution of lysosomes may be one of the criteria of cytoplasmic maturation of porcine oocytes. | |||||
書誌情報 |
Journal of mammalian ova research en : Journal of mammalian ova research 巻 26, 号 1, p. 48-53, 発行日 2009-04 |
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出版者 | ||||||
出版者 | 日本哺乳動物卵子学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13417738 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10548943 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1274/jmor.26.48 | |||||
権利 | ||||||
権利情報 | 本文データは学協会の許諾に基づきJ-Stageから複製したものである | |||||
著者版フラグ | ||||||
値 | publisher | |||||
異版である | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | https://www.jstage.jst.go.jp/article/jmor/26/1/26_1_48/_pdf |