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An Ultrastructural Study of Cell-Cell Contact between Mouse Spleen Cells and Calvaria-Derived Osteoblastic Cells in a Co-Culture System for Osteoclast Formation
http://hdl.handle.net/10191/5698
http://hdl.handle.net/10191/5698a8938fdd-c642-4903-9e2c-899b2af91585
名前 / ファイル | ライセンス | アクション |
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7_0003.pdf (3.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-04-08 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | An Ultrastructural Study of Cell-Cell Contact between Mouse Spleen Cells and Calvaria-Derived Osteoblastic Cells in a Co-Culture System for Osteoclast Formation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Co-culture | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Osteoclast formation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Osteoblastic cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell-cell contact | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Microenvironment | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Amizuka, Norio
× Amizuka, Norio× Takahashi, Naoyuki× Udagawa, Nobuyuki× Suda, Tatsuo× Ozawa, Hidehiro |
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著者別名 | ||||||
識別子 | 4015 | |||||
識別子Scheme | WEKO | |||||
姓名 | 網塚, 憲生 | |||||
言語 | ja | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have previously established a mouse co-culture system of osteoblastic cells and spleen cells for examining osteoclast differentiation. In the present study, we examined the morphological features of the cell-cell contact between mouse spleen cells and osteoblastic cells in the co-cultures. Light microscopic investigations revealed that tartrate-resistant acid phosphatase (TRAP)positive mononuclear and multinucleated spleen cells appeared in the vicinity of alkaline phosphatase (ALP)-positive osteoblastic cells. Ultrastructurally, spleen cells extended long cytoplasmic filopodia in all directions, by which spleen cells touched adjacent osteoblastic cells. The adjacent osteoblastic cells and spleen cells adhered to each other by forming electron-dense cytoplasmic materials on their inner leaflets of plasma membranes at cell-cell contact sites. Some adjacent osteoblastic and spleen cells made contact on the plasma membranes, forming an extracellular microenvironment. Coated pits were also formed on the plasma membranes facing this microenvironment. These morphological features of the cell-cell contact between osteoblastic cells and spleen cells indicate that there is internalization of organic components, i.e., receptor-mediated endocytosis in the contact sites between the two types of cells. | |||||
書誌情報 |
en : Acta histochemica et cytochemica 巻 30, 号 4, p. 351-362, 発行日 1997 |
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出版者 | ||||||
言語 | ja | |||||
出版者 | 日本組織細胞化学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0044-5991 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508022 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.1267/ahc.30.351 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者版フラグ | ||||||
値 | publisher |