WEKO3
アイテム
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C57BL/6マウスES細胞を用いたプルキンエ細胞および時期特異的遺伝子組換え系の開発
http://hdl.handle.net/10191/48356
http://hdl.handle.net/10191/483560f5c7fcd-3fc7-483c-b46b-d5aed3816a6f
名前 / ファイル | ライセンス | アクション |
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115(8)_397-407.pdf (5.7 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-11-09 | |||||
タイトル | ||||||
タイトル | C57BL/6マウスES細胞を用いたプルキンエ細胞および時期特異的遺伝子組換え系の開発 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | C57BL/6マウスES細胞を用いたプルキンエ細胞および時期特異的遺伝子組換え系の開発 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cerebellar Purkinje cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | spatio-temporally restricted gene targeting | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | C57BL/6 mouse | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ES cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | antiprogesterone | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 小脳プルキンエ細胞 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 部位・時期特異的ジーンターゲティング | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | C57BL/6マウス | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 胚性幹細胞 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | アンチプロゲステロン | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | Purkinje Cell Specific and Inducible Gene Recombination System Generated from C57BL/6 Mouse ES Cells | |||||
著者 |
北山, 和子
× 北山, 和子 |
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著者別名 | ||||||
識別子 | 95258 | |||||
識別子Scheme | WEKO | |||||
姓名 | Kitayama, Kazuko | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Spatio-temporally restricted gene targeting is needed for analyzing the functions of various molecules in a variety of biological phenomena. We have generated an inducible cerebellar Purkinje cell-specific gene targeting system. This was achieved by establishing a mutant mouse line (D2CPR) from a C57BL/6 mouse ES cell line, which expressed a fusion protein consisting of the Cre recombinase and the progesterone receptor (CrePR). The Purkinje cell-specific expression of CrePR was attained by inserting CrePR into the glutamate receptor 62 subunit (GluR δ2) gene, which was expressed specifically in the Purkinje cells. Using the transgenic mice carrying the Cre-mediated reporter gene expression, We showed that the antiprogesterone RU486 could induce recombinase activity of the CrePR protein specifically in the mature cerebellar Purkinje cells of the D 2 CPR line. Thus this mutant line will be a useful tool for studying the molecular function of mature Purkinje cells by manipulating gene expression in a temporally restricted manner. | |||||
書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 115, 号 8, p. 397-407, 発行日 2001-08 |
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出版者 | ||||||
出版者 | 新潟医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00290440 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00182415 | |||||
著者版フラグ | ||||||
値 | publisher |