WEKO3
アイテム
{"_buckets": {"deposit": "1dabfee3-1ca4-461d-ae92-a1ca1083e6e4"}, "_deposit": {"id": "2026", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "2026"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00002026", "sets": ["454", "484"]}, "author_link": ["6684", "6685", "6686", "6687", "6688"], "control_number": "2026", "item_1627363077551": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1995-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "207", "bibliographicPageStart": "193", "bibliographicVolumeNumber": "182", "bibliographic_titles": [{"bibliographic_title": "Journal of Immunological Methods", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We developed an improved technique that permits simultaneous DNA and RNA quantitation by a flow cytofluorometry using 7-amino-actinomycin D (7AAD) and pyronin Y (PY), respectively. Detailed cell cycle analyses based upon the cellular DNA/RNA levels were performed using cells suspended in a buffer containing 0.004% saponin. This method preserved the light scattering properties of human peripheral blood cells, thus lymphocyte, monocyte and granulocyte populations could be evaluated. In addition, since 7AAD and PY exhibit red (>650 nm) and orange fluorescence (570 nm) respectively, the green fluorescence channel of the flowcytometer was reserved for surface phenotyping using FITC-conjugated antibodies. The 7AAD/PY method is applicable to the simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation when combined with FITC-conjugated surface markers in heterogeneous samples. To demonstrate the three color analysis, PHA-activated human peripheral blood lymphocytes were stained for cell surface markers with monoclonal antibodies. The cells were suspended in buffer containing 0.004% saponin, then stained with 7AAD and PY. The DNA and RNA were analyzed in indivisual CD4+, CD8+ and CD20+ cells, and the characteristic cell cycle status was found. Cell activation was further analyzed using antibodies against IL-2 receptors (CD25), transferrin receptors (CD71) or HLA-DR molecules. Transferrin receptors were expressed in late G1 phase (G1B) just before the initiation of DNA synthesis, whereas IL-2 receptors and HLA-DR were expressed very early in the G1 phase (G1T). Since this technique preserves both light scatter properties as well as cell surface proteins, it is ideally suited for detailed cell cycle analyses of heterogeneous samples such as peripheral blood or bone marrow cells.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_5_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "6688", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "鳥羽, 健", "nameLang": "ja"}]}]}, "item_5_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_5_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://doi.org/10.1016/0022-1759(95)00050-k", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Elsevier B. V."}]}, "item_5_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_5_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00699645", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0022-1759", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Toba, Ken", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6684", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Winton, Elliott F.", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6685", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Koike, Tadashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6686", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shibata, Akira", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6687", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "24_0024.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "24_0024.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/2026/files/24_0024.pdf"}, "version_id": "f3c9cb31-c63c-48c1-a762-18b364870016"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "cell kinetics", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DNA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "RNA quantitation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "7-amino-actinomycin D", "subitem_subject_scheme": "Other"}, {"subitem_subject": "pyronin Y", "subitem_subject_scheme": "Other"}, {"subitem_subject": "saponin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Flow cytometry", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-Amino-actinomycin-D and Pyronim Y", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-Amino-actinomycin-D and Pyronim Y", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["454", "484"], "permalink_uri": "http://hdl.handle.net/10191/1168", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-04-23"}, "publish_date": "2007-04-23", "publish_status": "0", "recid": "2026", "relation": {}, "relation_version_is_last": true, "title": ["Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-Amino-actinomycin-D and Pyronim Y"], "weko_shared_id": -1}
Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-Amino-actinomycin-D and Pyronim Y
http://hdl.handle.net/10191/1168
http://hdl.handle.net/10191/116813a382c8-9a45-441c-a705-f8a1e83b0d35
名前 / ファイル | ライセンス | アクション |
---|---|---|
24_0024.pdf (1.1 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2007-04-23 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-Amino-actinomycin-D and Pyronim Y | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cell kinetics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RNA quantitation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 7-amino-actinomycin D | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | pyronin Y | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | saponin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Flow cytometry | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Toba, Ken
× Toba, Ken× Winton, Elliott F.× Koike, Tadashi× Shibata, Akira |
|||||
著者別名 | ||||||
識別子 | 6688 | |||||
識別子Scheme | WEKO | |||||
姓名 | 鳥羽, 健 | |||||
言語 | ja | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We developed an improved technique that permits simultaneous DNA and RNA quantitation by a flow cytofluorometry using 7-amino-actinomycin D (7AAD) and pyronin Y (PY), respectively. Detailed cell cycle analyses based upon the cellular DNA/RNA levels were performed using cells suspended in a buffer containing 0.004% saponin. This method preserved the light scattering properties of human peripheral blood cells, thus lymphocyte, monocyte and granulocyte populations could be evaluated. In addition, since 7AAD and PY exhibit red (>650 nm) and orange fluorescence (570 nm) respectively, the green fluorescence channel of the flowcytometer was reserved for surface phenotyping using FITC-conjugated antibodies. The 7AAD/PY method is applicable to the simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation when combined with FITC-conjugated surface markers in heterogeneous samples. To demonstrate the three color analysis, PHA-activated human peripheral blood lymphocytes were stained for cell surface markers with monoclonal antibodies. The cells were suspended in buffer containing 0.004% saponin, then stained with 7AAD and PY. The DNA and RNA were analyzed in indivisual CD4+, CD8+ and CD20+ cells, and the characteristic cell cycle status was found. Cell activation was further analyzed using antibodies against IL-2 receptors (CD25), transferrin receptors (CD71) or HLA-DR molecules. Transferrin receptors were expressed in late G1 phase (G1B) just before the initiation of DNA synthesis, whereas IL-2 receptors and HLA-DR were expressed very early in the G1 phase (G1T). Since this technique preserves both light scatter properties as well as cell surface proteins, it is ideally suited for detailed cell cycle analyses of heterogeneous samples such as peripheral blood or bone marrow cells. | |||||
言語 | en | |||||
書誌情報 |
en : Journal of Immunological Methods 巻 182, 号 2, p. 193-207, 発行日 1995-02 |
|||||
出版者 | ||||||
言語 | en | |||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0022-1759 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00699645 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.1016/0022-1759(95)00050-k | |||||
権利 | ||||||
権利情報 | Elsevier B. V. | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者版フラグ | ||||||
値 | author |