We studied the production of IL-1β, TNF-α and IFN-γ by alveolar cells obtained by bronchoalveolar lavage (BAL) in patients with sarcoidosis. The culture supernatant of alveolar macrophages (AMs) and peripheral blood monocytes (PB-Mo) of patients with sarcoidosis and control subjects were assayed for IL-1β and TNF-α using ELISA system. IFN-γ was also measured in the culture supernatants of BAL-lymphocytes (BAL-Ly) and peripheral blood lymphocytes (PB-Ly) by RIA system. In patients with sarcoidosis, the production of IL-1β was significantly higher in both LPS-stimulated AMs and PB-Mo than untreated AMs and PB-Mo. The content of TNF-α was significantly higher in the supernatant from lipopolysaccharide (LPS) stimulated AMs than that from untreated AMs. However, there was no significant difference in TNF-α content of the supernatant between LPS-stimulated and untreated PB-Mo. Concerning IFN-γ, the culture supernatant from both phytohemagglutinin stimulated BAL-Ly and PB-Ly contained more IFN-γ than in untreated BAL-Ly and PB-ly. This study demonstrated that AMs from patients with sarcoidosis increased the production of TNF-α and that this increase was compartmentalized to the lungs, since there was significant difference in TNF-α production between LPS-stimulated AMs and PB-Mo. It is suggested that acceleratod TNF-α release by AMs play a role in immune effector cells activation in the pulmonary sarcoidosis lesions.
サルコイドーシスにおける肺胞マクロファージIL-1β, TNF-αおよび肺胞リンパ球IFN-γの産生
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