WEKO3
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Chitinases A, B, and C1 from Serratia marcescens 2170 produced by recombinant Escherichia coli: enzymatic properties and synergism on chitin degradation
http://hdl.handle.net/10191/6328
http://hdl.handle.net/10191/632832603547-22d3-42c1-8b42-48f86dd9c005
名前 / ファイル | ライセンス | アクション |
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07_0004.pdf (499.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-04-23 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Chitinases A, B, and C1 from Serratia marcescens 2170 produced by recombinant Escherichia coli: enzymatic properties and synergism on chitin degradation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Serratia marcescens | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chitinase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | synergism | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Suzuki, Kazushi
× Suzuki, Kazushi× Sugawara, Noriko× Suzuki, Megumi× Uchiyama, Taku× Katouno, Fuminori× Nikaidou, Naoki× Watanabe, Takeshi |
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著者別名 | ||||||
識別子 | 4322 | |||||
識別子Scheme | WEKO | |||||
姓名 | 鈴木, 一史 | |||||
言語 | ja | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To discover the individual roles of the chitinases from Serratia marcescens 2170, chitinases A, B, and C1 (ChiA, ChiB, and ChiC1) were produced by Escherichia coli and their enzymatic properties as well as synergistic effect on chitin degradation were studied. All three chitinases showed a broad pH optimum and maintained significant chitinolytic activity between pH 4 and 10. ChiA was the most active enzyme toward insoluble chitins, but ChiC1 was the most active toward soluble chitin derivatives among the three chitinases. Although all three chitinases released (GlcNAc)2 almost exclusively from colloidal chitin, ChiB and ChiC1 split (GlcNAc)6 to (GlcNAc)3, while ChiA exclusively generated (GlcNAc)2 and (GlcNAc)4. Clear synergism on the hydrolysis of powdered chitin was observed in the combination between ChiA and either ChiB or ChiC, and the sites attacked by ChiA on the substrate are suggested to he different from those by either ChiB or ChiC1. | |||||
言語 | en | |||||
書誌情報 |
en : Bioscience, Biotechnology, and Biochemistry 巻 66, 号 5, p. 1075-1083, 発行日 2002 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0916-8451 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10824164 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.1271/bbb.66.1075 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者版フラグ | ||||||
値 | publisher |